Reactive oxygen species (ROS) is
produced in metabolic and physiological processes, and harmful oxidative
reactions may occur in organisms that remove them via enzymatic and
nonenzymatic antioxidative mechanisms. Under certain conditions, the increase
in oxidants and decrease in antioxidants cannot be prevented, and the oxidative
or in over 100 disorders, develops.
Antioxidant molecules prevent or inhibit
these harmful reactions. Serum (or plasma) concentrations of different
antioxidants can be measured in laboratories separately, but the measurements
are time-consuming, labor-intensive, costly, and require complicated techniques.
Because the measurement measurement of different antioxidant molecules
separately is not practical and their antioxidant effects are additive, the
total antioxidant capacity of a sample is measured, and this is called total
antioxidant capacity (TAC) , total antioxidant activity (TAA) , total
antioxidant power (TAOP) , total antioxidant status (TAS) , total
antioxidant response , or other synonyms.
In the test, antioxidants in the sample reduce
dark blue-green colored ABTS radical to colorless reduced ABTS form. The change
of absorbance at 660 nm is related with total antioxidant level of the sample.
The assay is calibrated with a stable antioxidant standard solution which is
traditionally named as Trolox Equivalent that is a vitamin E analog .
E Savas, N Aksoy, Y Pehlivan, ZA Sayiner… - Wiener klinische …, 2014 - Springer
… The TOS of serum was determined using a novel-automated measurement method, also
developed by Erel , the Rel Assay
Diagnostics kit, Mega Tip, Gaziantep, Turkey) …
TOS total oxidant status, TAS
status, OSI oxidative stress index … Cited by 14 Related articles All 6 versions
, H Bilgin
, F Çekmez, H Kurku… - Journal of clinical …, 2013 - ncbi.nlm.nih.gov
… Plasma total antioxidant
) and total oxidant status (TOS) level measurements (RELAssay
Diagnostics) were done in all participants … TAS
colorimetrically using the Total Antioxidant
Status kit (15) (REL Assay
Diagnostics) … Cited by 45 Related articles All 12 versions
H Toker, A Akpınar, H Aydın… - Journal of periodontal …, 2012 - Wiley Online Library
… TOS and TAS
levels were measured using commercially available kits (Rel Assay
, Mega Tıp,
Gaziantep, Turkey) by Erel's colorimetric method (21,22) at an … Table 3. Interleukin-1beta (IL-1β)
level, total oxidant status (TOS) and total antioxidant
) in gingival … Cited by 40 Related articles All 7 versions
G Özkan, S Ulusoy, M Alkanat, A Orem, B Akcan… - Renal failure, 2012 - Taylor & Francis
… Tissue TAS
, TOS, and OSI levels were assayed as previously described,11 using commercial
assay kits (Rel Assay
Diagnostics, Gaziantep … Notes: CsA, cyclosporine A; GSPE, grape seed
proanthocyanidin extract; TOS, total oxidant system; TAS
, total antioxidant
system; OSI … Cited by 24 Related articles All 3 versions
R Durgut, VS Ataseven, A Sağkan-Öztürk… - Journal of animal …, 2013 - academic.oup.com
… E and C increased antioxidant
capacity. Pagnini et al. (2004) indicated that antioxidants prevented
oxidative stress; however, they failed to stop virus growth. The objective of this study is to
demonstrate the relationship among total antioxidative status (TAS
), total oxidant status … Cited by 13 Related articles All 8 versions
E Taskin, N Dursun - Biological trace element research, 2012 - Springer
… Mitochondrial ATP content from each probe was assessed in duplicate. Biochemical Analysis.
Measurement of Total Antioxidant
Status. Total antioxidant
) of the cytosol and
of the kidney cell mitochondria were measured using Rel Assay
Kit … Cited by 26 Related articles All 10 versions
S Ozturk, O Ozcan
, AB Gurpinar, Y Can… - … journal of clinical and …, 2014 - ncbi.nlm.nih.gov
… from the cells by centrifugation at 3000 rpm for 15 min and were stored at -80°C and used for
the analysis of the Total Antioxidant
) and Total Oxidant Status (TOS). Analysis of
blood samples. Serum TAS
and TOS were determined with kits (Rel Assay
Diagnostics kit … Cited by 23 Related articles All 10 versions
, S Turkoglu, N Yildirim… - Digest Journal of …, 2012 - chalcogen.ro
… 2.7 Total Antioxidant
) Total antioxidant
status was determined by using Rel assay
assay kit (Lot.RL024) by Multiscan FC (Thermo). Antioxidants in the sample reduce
dark blue-green colored ABTS radical to colorless reduced ABTS form … Cited by 19 Related articles All 4 versions
K Kirboga, AV Ozec, M Kosker, A Dursun… - Journal of …, 2014 - hindawi.com
… equiv./L). 2.3.4. Total Antioxidant
Status Assay. TAS
was determined using commercialRel Assay
Diagnostic kits (Gaziantep, Turkey) with an autoanalyzer LX20-Pro from
Beckman-Coulter (Woerden, The Netherlands). The method … Cited by 9 Related articles All 12 versions
A Yağan, S Kesim, N Liman - Journal of periodontology, 2014 - Wiley Online Library
… Fully Automated 3rd Generation Total Antioxidant
) Assay Kit, Product Code RL0017,Rel Assay
Diagnostics, Mega Tıp, Gaziantep, Turkey. ## Fully Automated Total Oxidant Status
(TOS) Assay Kit, Product Code RL0024, Rel Assay
Diagnostics, Mega Tıp … Cited by 36 Related articles All 8 versions
|Reagent 1 - Buffer||1 x 30 ml|
|Reagent 2 - ABTS Radical Cation||1 x 8 ml|
|Standard||1 x 4 ml|
|Quality Control - Level 1||1 x 4 ml|
|Quality Control - Level 2||1 x 4 ml|
1. Halliwell B, Gutteridge JMC.
Free radicals in biology and medicine. Third ed. Oxford: Oxford Science
Publications; 2000. p. 617–24.
2. Young IS, Woodside JV.
Antioxidants in health and disease. J Clin Pathol 2001;54:176–86.
3. Erel O. A novel automated direct
measurement method for total antioxidant capacity using a new generation, more
stable ABTS radical cation. Clin Biochem. 2004 Apr;37(4):277-85.
4. Koracevic D, Koracevic G,
Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant
activity in human fluids. J Clin Pathol 2001;54(5):356 – 61.
5. Benzie IF, Strain JJ. The ferric
reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the
FRAP assay. Anal Biochem 1996;239:70– 6.
6. Aycicek A, Iscan A, Erel O,
Akcali M, Selek S. Total antioxidant/oxidant status in meningism and
meningitis. Pediatr Neurol. 2006 Dec;35(6):382-6.
7.Erel O. A novel automated method
to measure total antioxidant response against potent free radical reactions.
Clin Biochem. 2004 Feb;37(2):112-9.